Institut für Verfahrenstechnik, Umwelttechnik und Techn. Biowissenschaften
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CD-Laboratory Head Prof. Christoph Herwig

Gumpendorferstrasse 1a / 166-4 

A-1060 Wien, Österreich

emailto:christoph.herwig@tuwien.ac.at

Tel:     +43 1 58801 166400

Fax:    +43 1 58801 166980

Our METHODOLOGICAL APPROACH

The strategy to develop the methods in the CD Laboratory follows the method cycle: The objective of successful process development is the optimization of Quality Attributes (QA) and process variables such as product amount. Therefore experiments are carried out, varying set-points of process parameters (e.g. temperature, pH, feeding strategy) while process related variables (e.g. concentration of product, formation of carbon dioxide) are measured.

The method cycle as the central tool for the execution of the CD laboratory tasks.



The process parameters remain initially unknown to achieve this objective. Therefore the relationship between process parameters and process variables need to found in an iterative inverse analysis. This starts with process monitoring including analytical development and methods to check data consistency in order to achieve a reliable data set (Error: Reference source not foundupper right part). Subsequently, the data are to converted into information, in form of physiological descriptors such as yields and specific rates. Those descriptors are independent of initial conditions and scale. Those descriptors are used as a basis for experimental design, for example setting up strategies to analyze the effect of a specific rate constant on process performance and quality attributes (upper left side).

This iterative approach leads to the formulation of mechanistic hypotheses, which can be implemented in a model (lower left side). Finally predictive processing and reaching the objective function based on a complex set of process parameters is achieved by the implementation of the model and real-time control strategies (lower right side).

This cycle has therefore central importance for the entire execution of the CD laboratory:

  • It represents the basis to identify which methods needs to be developed.

  • It clearly demonstrates that the method development has to follow a counterclockwise sequential approach starting from the upper right side by developing analytical methods and setting up monitoring strategies.

  • Which methods need to be developed remains different for the individual research topic of course.

  • The method cycle including a systematic approach displays the core competence of the group of biochemical engineering at TU Wien. This competence, and the methodological synergies also allow us to executing research topics with different host organisms inside of the same CD laboratory.