Institut für Verfahrenstechnik, Umwelttechnik und Techn. Biowissenschaften
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Rastädter K, Wurm DJ, Spadiut O, Quehenberger J. Physiological Characterization of Sulfolobus acidocaldarius in a Controlled Bioreactor Environment. International Journal of Environmental Research and Public Health. 2021; 18(11):5532.

Kittler, S.; Slouka, C.; Pell, A.; Lamplot, R.; Besleaga, M.; Ablasser, S.; Herwig, C.; Spadiut, O.; Kopp, J. Cascaded processing enables continuous upstream processing with E. coli BL21(DE3). Scientific Reports 2021, 11, 11477, doi:10.1038/s41598-021-90899-9.

Kittler, S.; Besleaga, M.; Ebner, J.; Spadiut, O. Protein L—More Than Just an Affinity Ligand. Processes 2021,9, 874.

Ebner, J.; Humer, D.; Klausser, R.; Rubus, V.; Pell, R.; Spadiut, O.; Kopp, J. (2021) At-Line Reversed Phase Liquid Chromatography for In-Process Monitoring of Inclusion Body Solubilization. Bioengineering, 8, 78.

Doppler, P., Kriechbaum, R., Singer, B. Spadiut, O. (2021) Make microalgal cultures axenic again – a fast and simple workflow utilizing fluorescence-activated cell sorting. J. Microbiol. Methods, 186, 106256, doi:10.1016/j.mimet.2021.106256

Doppler P., Kornpointner C., Halbwirth H., Remias D., Spadiut O. (2021) Tetraedron minimum, First Reported Member of Hydrodictyaceae to Accumulate Secondary Carotenoids. Life, 11, 107. DOI: 10.3390/ life11020107

Kastenhofer J., Cataldo A.L., Ebner J., Sedlmayr V.L., Jungbauer A., Spadiut O. (in press) Economic and ecological benefits of a leaky E. coli strain for downstream processing: a case study for staphylococcal protein A. Journal of Chemical Technology & Biotechnology, doi: 10.1002/jctb.6691

Kastenhofer J., Rajamanickam V., Libiseller-Egger J., Spadiut O. (2021) Monitoring and control of E. coli cell integrity. Journal of Biotechnology 329, 1-12.

Schottroff F., Kastenhofer J., Jaeger H., Spadiut O., Wurm D.J. (2021) Selective release of recombinant periplasmic protein from E. coli using continuous pulsed electric field treatment. Frontiers in Bioengineering and Biotechnology 8, 586833.


Kopp, J., Kittler, S., Slouka, C., Herwig, C., Spadiut, O., & Wurm, D. J. (2020) Repetitive Fed-Batch: A Promising Process Mode for Biomanufacturing With E. coli. Frontiers in Bioengineering and Biotechnology 8, 573607.

Pekarsky, A., Spadiut, O., (2020) Intrinsically Magnetic Cells: A Review on Their Natural Occurrence and Synthetic Generation. Frontiers in Bioengineering and Biotechnology 8, 573183.

Eggenreich, B., Wurm, D.J., Rajamanickam, V., Klausser, R., Slouka, C., Spadiut, O., (2020) High pressure homogenization is a key unit operation in inclusion body processing. Journal of Biotechnology: X 7, 100022.

Kastenhofer, J., Rettenbacher, L., Feuchtenhofer, L., Mairhofer, J., Spadiut, O., (2020) Inhibition of E. coli Host RNA Polymerase Allows Efficient Extracellular Recombinant Protein Production by Enhancing Outer Membrane Leakiness. Biotechnology Journal, 200274.

Horvat, J., Narat, M., Spadiut O., (2020) The effect of amino acid supplementation in an industrial Chinese Hamster Ovary process. Biotechnology Progress 36.

Kittler, S., Kopp, J., Veelenturf, P.G., Spadiut, O., Delvigne, F., Herwig, C., Slouka, C., (2020) The Lazarus Escherichia coli Effect: Recovery of Productivity on Glycerol/Lactose Mixed Feed in Continuous Biomanufacturing. Frontiers in Bioengineering and Biotechnology 8.

Humer, D., Ebner, J., Spadiut, O., (2020) Scalable high-performance production of recombinant horseradish peroxidase from E. Coli inclusion bodies. International Journal of Molecular Sciences 21, 1-20.

Brunauer, G.C., Spadiut, O., Gruber, A., Slouka, C., (2020) Yeast propagation control: Low frequency electrochemical impedance spectroscopy as an alternative for cell counting chambers in brewery applications. Chemosensors 8.

Rastädter, K., Wurm, D.J., Spadiut, O., Quehenberger, J., (2020) The cell membrane of Sulfolobus spp.—homeoviscous adaption and biotechnological applications. International Journal of Molecular Sciences 21.

Schwaighofer, A., Ablasser, S., Lux, L., Kopp, J., Herwig, C., Spadiut, O., Lendl, B., Slouka, C., (2020) Production of active recombinant hyaluronidase inclusion bodies from Apis mellifera in E. coli BL21(DE3) and characterization by FT-IR spectroscopy. International Journal of Molecular Sciences 21, 1-17.

Quehenberger, J., Pittenauer, E., Allmaier, G., Spadiut, O., (2020) The influence of the specific growth rate on the lipid composition of Sulfolobus acidocaldarius. Extremophiles 24, 413-420.

Spadiut, O., Gundinger, T., Pittermann, B., Slouka, C., (2020) Spatially resolved effects of protein freeze-thawing in a small-scale model using monoclonal antibodies. Pharmaceutics 12.

Doppler, P., Veiter, L., Spadiut, O., Herwig, C., Rajamanickam, V., (2020) A chemometric tool to monitor and predict cell viability in filamentous fungi bioprocesses using UV chromatogram fingerprints. Processes 8.

Kastenhofer, J., Spadiut, O., (2020) Culture medium density as a simple monitoring tool for cell integrity of Escherichia coli. Journal of Biotechnology: X 6.

Hausjell, J., Kutscha, R., Gesson, J.D., Reinisch, D., Spadiut, O., (2020) The effects of lactose induction on a plasmid-free E. coli T7 expression system. Bioengineering 7.

Quehenberger, J., Steudler, S., Spadiut, O., (2020) Evaluation of the potential of an enzymatically treated beech wood hydrolysate as carbon source for Sulfolobus acidocaldarius. Bioresource Technology Reports 9.

Hausjell, J., Schendl, D., Weissensteiner, J., Molitor, C., Halbwirth, H., Spadiut, O., (2020) Recombinant production of a hard-to-express membrane-bound cytochrome P450 in different yeasts—Comparison of physiology and productivity. Yeast 37, 217-226.

Gundinger, T., Spadiut, O., (2020) pH conditioning is a crucial step in primary recovery - A case study for a recombinant Fab from E. coli. Protein Expression and Purification 165.


Hausjell, J., Miltner, M., Herzig, C., Limbeck, A., Saracevic, Z., Saracevic, E., Weissensteiner, J., Molitor, C., Halbwirth, H., Spadiut, O., (2019) Valorisation of cheese whey as substrate and inducer for recombinant protein production in E. coli HMS174(DE3). Bioresource Technology Reports 8.

Pekarsky, A., Konopek, V., Spadiut, O., (2019) The impact of technical failures during cultivation of an inclusion body process. Bioprocess and Biosystems Engineering 42, 1611-1624.

Kopp, J., Slouka C, Spadiut O and Herwig C (2019) The Rocky Road From Fed-Batch to Continuous Processing With E. coli. Front. Bioeng. Biotechnol. 7:328.

Kopp, J., Kolkmann, A.-M., Veleenturf, P.G., Spadiut, O., Herwig, C., and Slouka, C. (2019). Boosting Recombinant Inclusion Body Production—From Classical Fed-Batch Approach to Continuous Cultivation. Frontiers in Bioengineering and Biotechnology 7, 297.

Quehenberger, J., Albersmeier, A., Glatzel, H., Hackl, M., Kalinowski, J., Spadiut, O., (2019) A defined cultivation medium for Sulfolobus acidocaldarius. Journal of Biotechnology 301, 56-67.

Humer, D., Spadiut, O., (2019) Improving the performance of horseradish peroxidase by site-directed mutagenesis. International Journal of Molecular Sciences 20.

Wurm, D.J., Spadiut, O., (2019) Purification of recombinant glycoproteins from Pichia pastoris culture supernatants. Methods in Molecular Biology, pp. 343-350.

Slouka, C., Kopp, J., Strohmer, D., Kager, J., Spadiut, O., and Herwig, C. (2019). Monitoring and control strategies for inclusion body production in E. coli based on glycerol consumption. J Biotechnol 296, 75-82.

Wurm, D.J., Spadiut, O., (2019) Efficient development of a mixed feed process for Pichia pastoris. Methods in Molecular Biology, pp. 323-333.

Pekarsky, A., Spadiut, O., Rajamanickam, V., Wurm, D.J., (2019) A fast and simple approach to optimize the unit operation high pressure homogenization - A case study for a soluble therapeutic protein in E. coli. Preparative Biochemistry and Biotechnology 49, 74-81.

Slouka C, Kopp J., Spadiut O, Herwig C. Perspectives of inclusion bodies for bio-based products: curse or blessing?. Appl Microbiol Biotechnol. 2019;103(3):1143‐1153.

Quehenberger, J., Reichenbach, T., Baumann, N., Rettenbacher, L., Divne, C., Spadiut, O., (2019) Kinetics and predicted structure of a novel xylose reductase from Chaetomium thermophilum. International Journal of Molecular Sciences 20.


Hofer, A., Herwig, C., Spadiut, O., (2018) Lecithin is the key material attribute in soy bean oil affecting filamentous bioprocesses. AMB Express 8.

Pekarsky, A., Veiter, L., Rajamanickam, V., Herwig, C., Grünwald-Gruber, C., Altmann, F., Spadiut, O., (2018) Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: A morphological and physiological comparison. Microbial Cell Factories 17.

Humer, D., Spadiut, O., (2018) Wanted: more monitoring and control during inclusion body processing. World Journal of Microbiology and Biotechnology 34.

Hausjell, J., Weissensteiner, J., Molitor, C., Halbwirth, H., Spadiut, O., (2018) E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3). Microbial Cell Factories 17.

Eggenreich, B., Scholz, E., Wurm, D.J., Forster, F., Spadiut, O., (2018) The production of a recombinant tandem single chain fragment variable capable of binding prolamins triggering celiac disease. BMC Biotechnology 18.

Gundinger, T., Pansy, A., Spadiut, O., (2018) A sensitive and robust HPLC method to quantify recombinant antibody fragments in E. coli crude cell lysate. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1083, 242-248.

Hausjell, J., Halbwirth, H., Spadiut, O., (2018) Recombinant production of eukaryotic cytochrome P450s in microbial cell factories. Bioscience Reports 38.

Wurm, D.J., Quehenberger, J., Mildner, J., Eggenreich, B., Slouka, C., Schwaighofer, A., Wieland, K., Lendl, B., Rajamanickam, V., Herwig, C., Spadiut, O., (2018) Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli. Applied Microbiology and Biotechnology 102, 667-676.


Quehenberger, J., Shen, L., Albers, S.V., Siebers, B., Spadiut, O., (2017) Sulfolobus - A potential key organism in future biotechnology. Frontiers in Microbiology 8.

Rajamanickam, V., Krippl, M., Herwig, C., Spadiut, O., (2017) An automated data-driven DSP development approach for glycoproteins from yeast. Electrophoresis 38, 2886-2891.

Rajamanickam, V., Metzger, K., Schmid, C., Spadiut, O., (2017) A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris. Microbial Cell Factories 16.

Eggenreich, B., Rajamanickam, V., Wurm, D.J., Fricke, J., Herwig, C., Spadiut, O., (2017) A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization. Microbial Cell Factories 16.

Wurm, D.J., Marschall, L., Sagmeister, P., Herwig, C., Spadiut, O., (2017) Simple monitoring of cell leakiness and viability in Escherichia coli bioprocesses—A case study. Engineering in Life Sciences 17, 598-604.

Gundinger, T., Spadiut, O., (2017) A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli. Journal of Biotechnology 248, 15-24.

Wurm, D.J., Hausjell, J., Ulonska, S., Herwig, C., Spadiut, O., (2017) Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli BL21(DE3) strains. Scientific Reports 7.

Wurm, D.J., Slouka, C., Bosilj, T., Herwig, C., Spadiut, O., (2017) How to trigger periplasmic release in recombinant Escherichia coli: A comparative analysis. Engineering in Life Sciences 17, 215-222.

Kubizek, F., Eggenreich, B., Spadiut, O., (2017) Status quo in antibody-drug conjugates-can glyco-enzymes solve the current challenges? Protein and Peptide Letters 24, 686-695.

Wurm, D.J., Herwig, C., Spadiut, O., (2017) How to determine interdependencies of glucose and lactose uptake rates for heterologous protein production with E. coli. Methods in Molecular Biology, pp. 397-408.

Rajamanickam, V., Wurm, D., Slouka, C., Herwig, C., Spadiut, O., (2017) A novel toolbox for E. coli lysis monitoring. Analytical and Bioanalytical Chemistry 409, 667-671.


Wurm, D.J., Veiter, L., Ulonska, S., Eggenreich, B., Herwig, C., Spadiut, O., (2016) The E. coli pET expression system revisited—mechanistic correlation between glucose and lactose uptake. Applied Microbiology and Biotechnology 100, 8721-8729.

Bonifert, G., Folkes, L., Gmeiner, C., Dachs, G., Spadiut, O., (2016) Recombinant horseradish peroxidase variants for targeted cancer treatment. Cancer Medicine 5, 1194-1203.

Eggenreich, B., Willim, M., Wurm, D.J., Herwig, C., Spadiut, O., (2016) Production strategies for active heme-containing peroxidases from E. coli inclusion bodies - A review. Biotechnology Reports 10, 75-83.


Capone, S., Ćorajević, L., Bonifert, G., Murth, P., Maresch, D., Altmann, F., Herwig, C., Spadiut, O., (2015) Combining protein and strain engineering for the production of Glyco-engineered horseradish peroxidase C1A in Pichia pastoris. International Journal of Molecular Sciences 16, 23127-23142.

Capone, S., Horvat, J., Herwig, C., Spadiut, O., (2015) Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter. Microbial Cell Factories 14.

Gmeiner, C., Spadiut, O., (2015) Protein production with a Pichia pastoris OCH1 knockout strain in fed-batch mode. Glyco-Engineering: Methods and Protocols, pp. 91-101.

Krainer, F.W., Capone, S., Jäger, M., Vogl, T., Gerstmann, M., Glieder, A., Herwig, C., Spadiut, O., (2015) Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris. Microbial Cell Factories 14.

Gmeiner, C., Saadati, A., Maresch, D., Krasteva, S., Frank, M., Altmann, F., Herwig, C., Spadiut, O., (2015) Development of a fed-batch process for a recombinant Pichia pastoris strain expressing a plant peroxidase. Microbial Cell Factories 14.

Gmeiner, C., Spadiut, O., (2015) Effects of different media supplements on the production of an active recombinant plant peroxidase in a Pichia pastoris Δoch1 strain. Bioengineered 6, 175-178.

Gmeiner, C., Spadiut, O., (2015) Protein production with a Pichia pastoris OCH1 knockout strain in fed-batch mode. Methods in Molecular Biology, pp. 91-101.

Spadiut, O., Herwig, C., (2015) Microbes for the production of monoclonal antibodies and antibody fragments. BioSpektrum 21, 281-283.